Military Institute of Hygiene and Epidemiology


Military Institute of Hygiene and Epidemiology, Poland

Military Institute of Hygiene and Epidemiology (MIHE) is a unique research institute within the scale of the country and the Ministry of National Defence of Poland, which in terms of its activity, experienced personnel, and organization is prepared to conduct research works and trainings, take countermeasures and provide services for the MND and other state institutions in the field of health effects and ways to prevent diseases caused by Weapon of Mass Destruction Agents:




nuclear and non-ionizing radiation

In this regard, the Institute conducts its activities in accordance with the recommendations and standards of the North Atlantic Treaty Organization, and cooperates with the members of NATO and the European Union in establishing an international system against WMD. MIHE is a well-regarded, opinion-forming research center in domain of defence against biological weapons


Scientific Laboratories of The Military Institute of Hygiene and Epidemiology, found in 3 locations:

  1. Warszawa, ul. Kozielska 4

-Administration and management,

-Department of Hygiene and Physiology,

-Department of Microbiology,

-Laboratory of Epidemiology.

  1. Warszawa, ul. Szaserów 128

-Department of Pharmacology and Toxicology,

-Department of Microwave Safety,

-Department of Radiobiology and Radiation Protection,

-Department of Regenerative Medicine.

  1. Puławy, ul. Lubelska 2

-Biological Threats Identification and Countermeasure Centre


The Centre disposes BSL-3 laboratory in which bacteria causing diseases such as anthrax, plague, cholera, or tularaemia are examined.


In the AEDNet 7 (Marie Curie Action) Grant, Bacteriophage Team of Biological Threats Identification and Countermeasure Centre in Puławy has been engaged.


The bacteriophage Team of MIHE consists of five scientific persons and one technician:

– Romuald Gryko Ph.D., M.V.D., (specialist II0 in the field of clinical microbiology) as a group leader, currently manages of domestic grant funded by Ministry of Science and Higher Education and has a part in two grants funded by The National Centre for Research and Development. The projects concern EHEC bacteriophages and application of bacteriophages for water purification.

– Lidia Mizak Ph.D., (specialist I0 in the field of clinical microbiology) is experienced microbiologist and at the present she is involved in studies of P. aeruginosa phages.

-M.Sc. Aleksandra Kwiatek is microbiologist and in the past she was involved in transformation of Nicotiana tabacum (Warsaw University), and now in recombination of phage endolysines, and in mechanisms of growth of biofilms.

-M.Sc. Sylwia Parasion is biotechnologist and she is engaged in studies of phage enzymes recombination and application of phages to destroy of biofilms (A. baumani).

-M.Sc. Aleksandra Nakonieczna is biotechnologist and she currently characterizes EHEC and Bacillus sp. bacteriophages.

-Mrs Ewa Jonska is technicians in our laboratory.


Bacteriophage Laboratory mainly works with bacteriophages of bacteria crucial in wounds infections ( A. baumani, E. fecalis, P. aeruginosa or MRSA) but also with lytic bacteriophages of B. anthracis and EHEC strains. Our scientific interests concentrate on phage enzymes (lysines and depolimerases) and possibility of their application.

We executed one DARPA grant concerning of recombinant endolysines against MRSA and VRE and other domestic grants concerning bacteriophages S. aureus and Enterococcus spp.

Relevant publications of our group:

-Magdalena Kwiatek , Lidia Mizak, Sylwia Parasion, Romuald Gryko, Alina Olender & Marcin Niemcewicz: Characterization of five newly isolated bacteriophages active

against Pseudomonas aeruginosa clinical strains, Folia Microbiol. July. 2014 DOI 10.1007/s12223-014-0333-3

-Negus D, Burton J, Sweed A, Gryko R, Taylor PW.:Poly-γ-D-glutamic acid capsule interferes with lytic infection of Bacillus anthracis by anthrax-specific bacteriophages. Appl Environ Microbiol. 2012 Nov 2. [Epub ahead of print]

-Parasion S, et al., Isolation and characterization of a novel bacteriophage φ4D lytic against Enterococcus faecalis strains. Curr Microbiol. 2012 Sep;65(3):284-9. Epub 2012 Jun 6.

-Magdalena Kwiatek et al., Characterization of a bacteriophage, isolated from a cow with mastitis, that is litic against Staphylococcus aureus strain. Arch. Virol. 2012, 157:225-234 .

-Agata Bielawska–Drózd et al., Detection of Enterohaemorrhagic Escherichia coli by Triplex Real Time PCR Method. Bull Vet Inst Pulawy 55, 635-639, 2011 .

-Janusz Kocik et al., Bacteriophages. Present and Future. Wellness in Different Phases of Life. 2011, May. 49.



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